Blue Loading Buffer Pack from Cell Signaling Technology

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ItemBlue Loading Buffer Pack
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7722S
Quantity1 Pack
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) An adaptive stress response that confers cellular resilience to decreased ubiquitinationNature CommunicationsNovember 14, 2023Liam C. Hunt, Vishwajeeth Pagala, Anna Stephan, Boer Xie, Kiran Kodali, Kanisha Kavdia, Yong-Dong Wang, Abbas Shirinifard, Michelle Curley, Flavia A. Graca, et al.(detergent-insoluble fraction) was retrieved, boiled with SDS-Blue loading buffer (Cell Signaling #7722) and DTT (Cell Signaling #1425 S), and analyzed by SDS-PAGE and western blot. The following antibodies

(2) USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stressNature CommunicationsOctober 13, 2023Kewei Qin, Shuhan Yu, Yang Liu, Rongtian Guo, Shiya Guo, Junjie Fei, Yuemeng Wang, Kaiyuan Jia, Zhiqiang Xu, Hu Chen, et al.For Western blot analyses, The insoluble fraction containing nucleoli was lysed in 1×SDS Sample Buffer (#7722, CST) and sonicated if necessary. For the co-immunoprecipitation assay, the nucleoli were lysed in high

(3) HIGD2A silencing impairs hepatocellular carcinoma growth via inhibiting mitochondrial function and the MAPK/ERK pathwayJournal of translational medicineApril 12, 2023Huang K, Liu Z, Xie Z, Li X, Zhang H, Chen Y, Wang Y, Lin Z, Li C, Liu H, et al.(Genechem, China). Western blotting analysis Cell protein samples were lysed with loading buffer (7722, Cell Signaling Technology, USA) and were then heated at 100 °C for 10 min. Cell lysates were separated

(4) HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophagesbioRxiv : the preprint server for biologyMarch 21, 2023Virgilio MC, Disbennett WM, Chen T, Lubow J, Welch JD, Collins KL.captured at 4 °C overnight. Immunoblot Western blots: cells were lysed in Blue Loading Buffer (cat# 7722, Cell Signaling Technology), sonicated with a Misonix sonicator (Qsonica, LLC. Newtown, CT), boiled

(5) NAD+ precursor supplementation prevents mtRNA/RIG-I-dependent inflammation during kidney injuryNature MetabolismMarch 13, 2023Tomohito Doke, Sarmistha Mukherjee, Dhanunjay Mukhi, Poonam Dhillon, Amin Abedini, James G. Davis, Karthikeyani Chellappa, Beishan Chen, Joseph A. Baur, Katalin Susztakmouse kidney samples39. Western blot Mice kidney lysates were homogenized in SDS lysis buffer (CST, #7722) with 2-Mercaptoethanol, and boiled for 5 minutes at 95 degrees. Lysates were transferred onto polyvinylidene

(6) Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular InfectionmBioFebruary 28, 2023Ganesan S, Alvarez NN, Steiner S, Fowler KM, Corona AK, Roy CR.and Fig. S2A, cells were directly lysed using the 1× blue loading buffer (Cell Signaling Technology, 7722). Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes. The primary antibodies

(7) Production and Characterization of K562 Cellular Clones Hyper-Expressing the Gene Encoding α-Globin: Preliminary Analysis of Biomarkers Associated with AutophagyGenes (Basel)February 23, 2023Matteo Zurlo, Jessica Gasparello, Lucia Carmela Cosenza, Giulia Breveglieri, Chiara Papi, Cristina Zuccato, Roberto Gambari, Alessia Finottifor 5 min at 98 °C in a 1× SDS sample buffer (Cell Signalling Technology, Danvers, MA, USA, cat. n. 7722) and loaded on hand casted SDS-PAGE 14% polyacrylamide gel (10 cm × 8 cm) in Tris-glycine Buffer. The

(8) Increased levels of endogenous retroviruses trigger fibroinflammation and play a role in kidney disease developmentNature communicationsFebruary 2, 2023Dhillon P, Mulholland KA, Hu H, Park J, Sheng X, Abedini A, Liu H, Vassalotti A, Wu J, Susztak K.dsRNA content in SHAM and UUO kidneys. For dsRNA interaction with RIG-I, SDS lysis buffer (CST, Cat# 7722) was added to dsRNA bound beads and followed by western blot with RIG-I antibody. For reverse CO-IP,

(9) Modulation of protease expression by the transcription factor Ptx1/PITX regulates protein quality control during agingCell ReportsJanuary 31, 2023Jianqin Jiao, Michelle Curley, Flavia A. Graca, Maricela Robles-Murguia, Abbas Shirinifard, David Finkelstein, Beisi Xu, Yiping Fan, Fabio Demontisplates,Biorad,HSP9601 Transparent 96-well plates,Corning,REF3599 PBS,Gibco,10010023 Blue loading buffer pack,Cell Signaling,7722 Precision Plus protein standard,Bio-Rad,1610374 4–20% Mini-PROTEAN TGX pre-cast gels,Bio-Rad,4561096 Immobilon-P

(10) Single-cell analysis highlights differences in druggable pathways underlying adaptive or fibrotic kidney regenerationNature communicationsJuly 11, 2022Balzer MS, Doke T, Yang YW, Aldridge DL, Hu H, Mai H, Mukhi D, Ma Z, Shrestha R, Palmer MB, et al.15 mg of total kidney tissue was homogenized in SDS lysis buffer (Cell Signaling Technology, CST, #7722), sonicated, and heated at 95 °C. Lysates were cleared by centrifuging (14,000 × g at 4 °C for 10 min).

(11) Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate CancerCancer ResearchJuly 5, 2022Liang Qin, Yoon-Mi Chung, Michael Berk, Bryan Naelitz, Ziqi Zhu, Eric Klein, Abhishek A. Chakraborty, Nima Sharifikit (MK28) from Bertin Technologies following the manufacturer’s protocol. 3X blue loading buffer (7722) from Cell Signaling was added to the samples which were then boiled for 10min before being loaded on

(12) The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophyNature communicationsMay 2, 2022Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, et al.concentrate, #5000006). Protein samples were prepared by addition of SDS-Blue loading buffer (Cell Signaling, #7722) and DTT (dithiothreitol; to a final concentration of 0.1 M, Cell Signaling, CST#1425 S) and heating

(13) PXR activation impairs hepatic glucose metabolism partly via inhibiting the HNF4Î±â€“GLUT2 pathwayActa Pharmaceutica Sinica BMay 1, 2022Peihua Liu, Ling Jiang, Weimin Kong, Qiushi Xie, Ping Li, Xiaonan Liu, Jiayi Zhang, Ming Liu, Zhongjian Wang, Liang Zhu, et al.lysates were extracted using Blue Loading Buffer Pack (Cell Signaling Technology, Danvers, MA, USA, 7722S) containing 1 mmol/L phenylmethylsulfonyl fluoride (PMSF). Fifty micrograms of mouse liver tissue was

(14) Analysis of L Antigen Family Member 3 as a Potential Biomarker and Therapeutic Target Associated With the Progression of Hepatocellular CarcinomaFrontiers in oncologyJanuary 1, 2022Chen Q, Lu X, Xie J, Ma N, Xu W, Zhang Z, Huang X, Liu H, Hou J, Zhang X, et al.For immunoblotting, protein samples from cells in each group were collected with Blue Loading Buffer Pack (7722S, CST, USA). After heating at 100°C for 10 minutes, the protein lysates were separated by sodium dodecyl

(15) Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophyCell reportsNovember 9, 2021Hunt LC, Graca FA, Pagala V, Wang YD, Li Y, Yuan ZF, Fan Y, Labelle M, Peng J, Demontis F.serum,GIBCO,10437-028 Penicillin streptomycin,GIBCO,15140122 PBS,GIBCO,10010023 Blue loading buffer pack,Cell Signaling,7722 Precision Plus protein standard,Bio-Rad,1610374 4-20% Mini-PROTEAN TGX pre-cast gels,Bio-Rad,4561096 Immobilon-P

(16) Genome-wide association studies identify the role of caspase-9 in kidney diseaseScience advancesNovember 5, 2021Doke T, Huang S, Qiu C, Sheng X, Seasock M, Liu H, Ma Z, Palmer M, Susztak K.S4. Western blot Tissue lysates were homogenized in SDS lysis buffer [Cell Signaling Technology (CST), #7722] and transferred onto polyvinylidene difluoride membranes. After blocking in 5% milk in tris-buffered

(17) Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractionsSTAR ProtocolsSeptember 17, 2021Mamta Rai, Michelle Curley, Zane Coleman, Anjana Nityanandam, Jianqin Jiao, Flavia A. Graca, Liam C. Hunt, Fabio DemontisSDS-PAGE Protein samples are combined with SDS blue loading buffer containing DTT (Cell signaling #7722, as per user’s manual) and boiled at 95°C for 5 min.18.The samples are centrifuged briefly to collect

(18) Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal CancerNature communicationsJuly 21, 2021Wang W, Tang YA, Xiao Q, Lee WC, Cheng B, Niu Z, Oguz G, Feng M, Lee PL, Li B, et al.volumes of proteins were mixed with one volume of 3 × Blue Loading Buffer (Cell Signaling Technology #7722) and boiled at 95 °C for 5 min. Proteins were run on pre-cast gels (Bio-Rad #4568096 or #4568086) or

(19) Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascadeCell death and differentiationJuly 1, 2021Wang W, Yan T, Guo W, Niu J, Zhao Z, Sun K, Zhang H, Yu Y, Ren T.after centrifugation, samples were boiled for 10 min after the addition of 40 μl 3 × loading buffer (#7722, CST). For WB, proteins were separated by SDS-PAGE gel electrophoresis and wet-transferred onto PVDF

(20) Role of c-Met/β1 integrin complex in the metastatic cascade in breast cancerJCI InsightJune 22, 2021Darryl Lau et al.magnetic rack and resuspending in 40 μL of 3× Blue Sample Buffer 30× Reducing Agent: 1.25 M DTT (1:30; 7722s, Cell Signaling Technology). Resulting samples were heated at 95°C (5 minutes), centrifuged at 300g

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Blue Loading Buffer Pack from Cell Signaling Technology

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Blue Loading Buffer Pack from Cell Signaling Technology

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